Search for LT-II and STb DNA Sequences Among Escherichia coli Isolated from Bovine Meat Products by Colony Hybridization
Authors: Cerqueira, Aloysio M.F.1; Tibana, Anita1; Gomes, Tania A.T.2; Guth, Beatriz E.C.2
Source: Journal of Food Protection®, Volume 57, Number 8, August 1994 , pp. 734-736(3)
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Abstract:
A total of 1,066 Escherichia coli colonies isolated from 105 raw bovine meat samples purchased at supermarkets in Rio de Janeiro were submitted to hybridization assays with gene probes for LT-II and STb enterotoxins. Five colonies comprising four different E. coli strains isolated from four pieces of beef, two samples of ground beef (5.7%) and two hamburger patties (5.7%) hybridized with the LT-II probe, while no hybridization occurred with the STb probe. Expression of LT-II enterotoxin using the Y1 adrenal cell assay was verified in two of four E. coli strains. A serotype diversity existed among LT-II E. coli strains.Keywords: LT-II; STB; ESCHERICHIA COLI; BOVINE MEAT
Document Type: Research article
Affiliations: 1: lnstituto de Microbiologia, Departamento de Microbiologia Medica, Universidade Federal do Rio de Janeiro - CCS - Bloco I, CEP: 21941, Brazil 2: Departamento de Microbiologia, Imunologia, Parasitologia, Escola Paulista de Medicina, CEP: 04023-062, Brazil
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