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Mycelial growth of Aspergillus parasiticus NRRL 2667 and aflatoxin production on Florunner peanuts grown under different calcium supplementation levels (CSL) (550, 1,100, 2,200 and 4,400 kg gypsum/ha) with initial spore loads of 102, 104 and 106
spores/g were investigated. Growth at 25°C for 0, 4, 7 and 14 days was determined by viable plate counts on Aspergillus flavus/parasiticus agar (AFPA) medium. Irrespective of the initial spore load, maximum growth of 108 to 109 CFU/g was attained after 14 days
except for the 4,400 kg/ha Ca-supplemented nuts on which the maximum populations were one log less. Aflatoxins B1, and G1 concentrations measured by thin layer chromatography (TLC) ranged from 0-3460 μg/g and 0-3740 μg/g, respectively. Toxin production was highest
in single peanuts with CSL of 2,200 kg/ha. A reduction of 50% or higher was observed as CSL was increased to 4,400 kg/ha.
Department of Food Science and Technology, University of Georgia, Athens, Georgia 30602 2:
Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223
Publication date: May 1, 1994
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