Antibacterial Activity of the Lactoperoxidase System Against Salmonella typhimurium in Trypticase Soy Broth in the Presence and Absence of a Heat Treatment
Abstract:The antibacterial activity of the lactoperoxidase system (LP system) on the growth and survival of Salmonella typhimurium in tryptic soy broth (TSB) was determined. The LP system, consisting of lactoperoxidase (1 μg/ml), potassium thiocynante (KSCN) (5.9 mM) and H2O2 (2.5 mM), was found to have both bacteriostatic and bactericidal activities against strains of S. typhimurium. The bactericidal activity was clearly dependent on the initial inoculum level. Levels of 102 − 105 CFU/ml were killed at 37°C as opposed to the bacteriostatic activity with inoculum levels of 106 − 107 CFU/ml, which exhibited a 25-h lag. The LP system also enhanced thermal inactivation of S. typhimurium. D50°C-values were > 60 min (untreated broth) and 12 min (LP system activated); corresponding D52°C-values were 20 min and 7.5 min; corresponding D55°C-values were 14 min and 4 min; corresponding D58°C-values were 5 min and 2.5 min and corresponding D60°C-values were .31 min and .20 min.
Document Type: Research Article
Affiliations: Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska 68583-0919
Publication date: May 1, 1994
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- ingentaconnect is not responsible for the content or availability of external websites