Polymerase Chain Reaction-Based DNA Fingerprinting for Identification of Streptococcus and Enterococcus Species Isolated from Bovine Milk
Abstract:A total of 353 isolates including 304 isolates from bovine milk belonging to 8 Streptococcus and Enterococcus species, 14 American Type Culture Collection reference strains, 10 isolates from bovine milk that had atypical biochemical profiles, and 25 bovine milk isolates from Vermont were examined by polymerase chain reaction-based DNA fingerprinting. A single short oligonucleotide primer of eight bases (5'-GTAACGCC-3') was used to produce a characteristic spectrum of amplified DNA fragments. Amplified DNA fragments were visualized following agarose gel electrophoresis and analyzed by scanning laser densitometry. Amplified DNA fragments were categorized as either primary, secondary, or variable fragments. DNA fragments observed in all isolates within a species were designated as primary [optical density > 0.3 absorbance units (AU)] and secondary fragments (optical density > 0.15 AU), while infrequent DNA fragments were designated as variable fragments (optical density > 0.15 AU). Examination of fingerprint patterns revealed that each of the species evaluated possessed a unique fingerprint profile. A simple identification scheme was developed based on the occurrence of primary and secondary DNA fragments for a species. The polymerase chain reaction-based DNA fingerprinting assay was simple to perform, reliable, and reproducible. Results of this study indicate that DNA fingerprinting using polymerase chain reaction has the potential to be developed as a routine method for species identification of bacteria.
Document Type: Research Article
Affiliations: Department of Animal Science, Institute of Agriculture, The University of Tennessee. Knoxville, Tennessee, 37901-1071
Publication date: March 1, 1994
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites