Standardized Method for Determining the Effect of Various Antibiotics on Lactococcal Cultures
Abstract:Antibiotic resistance of 44 lactic starter culture strains currently used by the dairy industry has been examined in Mueller-Hinton medium, supplemented with glucose (5 g/L) and yeast extract (5 g/L), by the agar disk diffusion assay. Results with various classes of antibiotics used to treat cattle mastitis indicated that lactococcal cultures should be diluted 20% and incubated at 30°C for 16 to 18 h in this medium for reliable results. The degree of antibiotic susceptibility was compared to standards established by the National Committee for Clinical Laboratory Standards. The procedure outlined in the present study is recommended as a routine method for testing antibiotic resistance of lactococci. This represents a more logical drug sensitivity approach than the use of noncheese starter bacteria to test for antibiotics in milk.
Document Type: Research Article
Affiliations: Department of Microbiology, Oregon State University, Corvallis Oregon 97331-3804
Publication date: March 1, 1994
More about this publication?
- IAFP Members with personal subscriptions to JFP Online: To access full-text JFP or JMFT articles, you must sign-in in the upper-right corner using your Ingenta sign-in details (your IAFP Member Login does not apply to this website). The Journal of Food Protection (JFP) is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.
Print and online subscriptions are available to IAFP Members and institutional subscribers. IAFP Members with a subscription to JFP Online will have access to all available JFP and JMFT content. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Membership and subscription information is available at www.foodprotection.org.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Ingenta Connect is not responsible for the content or availability of external websites