Ineffectiveness of Hot Acid Sprays to Decontaminate Escherichia coli 0157:H7 on Beef

Authors: Brackett, R.E.; Hao, Y.-Y.; Doyle, M.P.

Source: Journal of Food Protection®, Number 3, March 1994, pp. 184-266 , pp. 198-203(6)

Publisher: International Association for Food Protection

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Abstract:

The efficacy of warm (20°C) and hot (55°C) acetic, citric and lactic acid sprays on survival of Escherichia coli 0157:H7 on raw beef was determined. Fresh, raw beef sirloin tips were sliced into ca. 1-cm slices with a sanitized slicer. Discs (5-cm diameter, ca. 25-g) were then taken from internal areas of each slice and inoculated with a 5-strain mixture of E. coli 0157:H7 such that final populations on beef samples were 103 or 106 CFU/g. After standing for 15 min, each inoculated disc was sprayed with 1 ml of 0, 0.5, 1.0 and 1.5% of each acid at 20 or 55°C using an atomizer. Acid-treated meat samples were allowed to dry for 15 min and held at 5°C for up to 13 days in sterile plastic pouches. Escherichia coli 0157:H7 was acid tolerant, and acids did not differ appreciably in their lack of antimicrobial activity on E. coli 0157:H7. For example, reductions in populations differed by <0.3 log10 CFU/g immediately after treatment and < 0.5 log10 CFU/g after 13 days incubation, compared to untreated controls. None of the acid treatments appreciably reduced E. coli 0157:H7 on beef samples nor were any of the acid treatments judged effective for practical uses.

Document Type: Research Article

Affiliations: Center for Food Safety and Quality Enhancement, Georgia Station, University of Georgia, Griffin, Georgia 30223-1797

Publication date: March 1, 1994

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    First published in 1937, the Journal of Food Protection®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

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