Detecting Oxytetracycline Residues in Chicken Tissues Using the Delvotest^® P System

Authors: Bugyei, Kwasi; Black, William; McEwen, Scott; Meek, Allan H.

Source: Journal of Food Protection®, Volume 57, Number 2, February 1994 , pp. 141-145(5)

Publisher: International Association for Food Protection

Buy & download fulltext article:

Price: $37.00 plus tax (Refund Policy)

Abstract:

Oxytetracycline spiked chicken liver and kidney were tested for residues using Delvotest P and the results compared to standard plate assay using Bacillus cereus as test organism. Delvotest P detected all replicates at concentrations of ≥0.62 μg/g in liver and kidney. Between 0.41-0.2 μg/g, the responses were mixed (positive, doubtful, and negative); at 0.1 ≥ concentration, the responses were negative. The limit of detection of the plate assay was 0.2 and 0.31 μg/g for liver and kidney, respectively. Residue analysis on tissues from chickens dosed twice daily (for 4 d) with 25 mg/kg oxytetracycline was carried out using the Delvotest P and the plate assay. Delvotest P responses were all positive for 1-4 h after treatment in kidney and 1 and 2 h in liver and serum. Muscle never achieved concentrations that were consistently positive. The plate assay detected drug from 0.5-6 h in serum, from 0.5-8 h in liver and kidney, and at 4-6 h in muscle. Kidney tissue appeared to be the best for detecting oxytetracycline residues in chicken. The Delvotest P method was simpler to use and required less time; 3-4 h compared to about 18 h for the plate assay.

Document Type: Research article

Affiliations: 1: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, NlG 2WI Canada

Publication date: 1994-02-01

More about this publication?

IAFP members must first sign in on the right to access full text articles of JFP

First published in 1937, the Journal of Food Protection^®, is a refereed monthly publication. Each issue contains scientific research and authoritative review articles reporting on a variety of topics in food science pertaining to food safety and quality. The Journal is internationally recognized as the leading publication in the field of food microbiology with a readership exceeding 11,000 scientists from 70 countries. The Journal of Food Protection^® is indexed in Index Medicus, Current Contents, BIOSIS, PubMed, Medline, and many others.

Print and online subscriptions are available to Members and Institutional subscribers. Online visitors who are not IAFP Members or journal subscribers will be charged on a pay-per-view basis. Information can be obtained by calling +1 800.369.6337; +1 515.276.3344; fax: +1 515.276.8655, E-mail: info@foodprotection.org or Web site: www.foodprotection.org
Information for Authors
Submit a Paper
Subscribe to this Title
Membership Information
Information for Advertisers
ingentaconnect is not responsible for the content or availability of external websites