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Testing of Bob Calf Fecal Swabs for the Presence of Escherichia coli O157:H7

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Rectal swabs were collected from 304 Bob calves (calves under 10 d old) brought to slaughter in the states of Washington (77 swabs), California (127 swabs), and Wisconsin (100 swabs).The swab samples were tested for the presence of Escherichia coli O157:H7 by the use of a direct smear method, enrichment method,and the use of the Petrifilm Test Kit-HEC-for hemorrhagic E.coli O157:H7 (3M Company, St. Paul, MN). The organism was not isolated from any of the samples by any method, though the3M test kit did give 21 positive signals. Of these positive signals, three were shown to be caused by sorbitol-positive, O157-positive, H7-negative E. coli. The cause of the other 18 signals was not determined.

Document Type: Research Article

Affiliations: 1: Food Safety and Inspection Service, Western Laboratory, U.S. Department of Agriculture, P.O. Box 4008, Alameda, CA 94501-0408 2: Food Safety and Inspection Service, Process Products Inspection Division, Thermal Processing Branch. U. S. Department of Agriculture, 14th & Independene Ave., Washington, DC 20250 3: Agricultural Marketing Service, Science Division, U.S. Department of Agriculture, Box 96456, Washington, DC 20090-6456

Publication date: January 1, 1994

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