Abstract:

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and β-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.

Keywords: RNA isolation; CTAB; fruit tissue; bilberry; Vaccinium myrtillus

Document Type: Research article

DOI: 10.1385/MB:19:2:201

Affiliations: 1: Department of Biology, University of Oulu, P.O.Box 3000, FIN-90014, Oulu, Finland, Email: Laura.Jaakola@oulu.fi