Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit

Authors: Jaakola, Laura¹; Pirttilä, Anna; Halonen, Minna; Hohtola, Anja

Source: Molecular Biotechnology, Volume 19, Number 2, October 2001 , pp. 201-203(3)

Publisher: Humana Press

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Abstract:

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and β-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.

Keywords: RNA isolation; CTAB; fruit tissue; bilberry; Vaccinium myrtillus

Document Type: Research article

DOI: 10.1385/MB:19:2:201

Affiliations: 1: Department of Biology, University of Oulu, P.O.Box 3000, FIN-90014, Oulu, Finland, Email: Laura.Jaakola@oulu.fi

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