Tubulin is the target binding site for NAP-related peptides: ADNF-9, d-NAP, and d-SAL

Authors: Holtser-Cochav, Miri¹; Divinski, Inna¹; Gozes, Illana²

Source: Journal of Molecular Neuroscience, Volume 28, Number 3, October 2006 , pp. 303-307(5)

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Abstract:

The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-d-enantiomers, d-NAP and d-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.

Keywords: ADNF; d-NAP; d-SAL; NAP; tubulin; ADNP; VIP; neuroprotection

Document Type: Research article

DOI: http://dx.doi.org/10.1385/JMN:28:3:303

Affiliations: 1: Human Molecular Genetics & Biochemistry, Sackler Medical School, Tel Aviv University, Israel, 2: Human Molecular Genetics & Biochemistry, Sackler Medical School, Tel Aviv University, Israel, Email: igozes@post.tau.ac.il

Publication date: 2006-10-01