Molecular cloning and analysis of Ca2+/calmodulin-dependent protein kinase II from the chicken brain
Source: Journal of Molecular Neuroscience, Volume 11, Number 2, October 1998 , pp. 135-139(5)
Publisher: Humana Press
Abstract:The goals of this study were to identify specific mRNAs for isoforms of calmodulin-dependent protein kinase II in chicken forebrain, prepare a cDNA expression library, and perform a sequence analysis of the kinase cDNA. Specific mRNAs for α- and -subunits of the kinase were identified in Northern blots. The mRNA for the α-subunit is larger in the chicken than in the rat, and for the -subunit is smaller in the chicken than the rat. Nucleotide sequencing of selected clones demonstrated the presence of an α-subunit with a 33 nucleotide insert known as the α-B-isoform. Clones of the -subunit showed it to contain a deletion of six nucleotides relative to previously described sequences. Variability in the mRNAs of calmodulin kinase II, as shown here, reflect the presence of species-dependent variability in gene structure as well as the presence of different functional isoforms.
Keywords: Chicken forebrain; calmodulin kinase II isoform; calmodulin kinase II subunit; calmodulin kinase II α-B-isoform; calmodulin kinase II α-B-subunit; calmodulin kinase II -subunit; chicken calmodulin kinase II
Document Type: Research Article
Affiliations: 1: Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY, 2: Laboratory of Molecular Neurobiology, Cornell University Medical College at The Burke Medical Research Institute, White Plains, NY, 3: Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, 4: Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY,
Publication date: 1998-10-01