Calcium Signaling in Isolated Skeletal Muscle Fibers Investigated Under "Silicone Voltage-Clamp" Conditions

In this: publication
By this: publisher
In this Subject: Biology
By this author: Claude Collet ;&nbsp; Sandrine Pouvreau ;&nbsp; Laszlo Csernoch ;&nbsp; Bruno Allard ;&nbsp; Vincent Jacquemond

Authors: Claude Collet; Sandrine Pouvreau; Laszlo Csernoch; Bruno Allard; Vincent Jacquemond

Source: Cell Biochemistry and Biophysics, Volume 40, Number 2, April 2004 , pp. 225-236(12)

Buy & download fulltext article:

Price: $42.00 plus tax (Refund Policy)

Abstract:

In skeletal muscle, release of calcium from the sarcoplasmic reticulum (SR) represents the major source of cytoplasmic Ca²⁺ elevation. SR calcium release is under the strict command of the membrane potential, which drives the interaction between the voltage sensors in the t-tubule membrane and the calcium-release channels. Either detection or control of the membrane voltage is thus essential when studying intracellular calcium signaling in an intact muscle fiber preparation. The silicone-clamp technique used in combination with intracellular calcium measurements represents an efficient tool for such studies. This article reviews some properties of the plasma membrane and intracellular signals measured with this methodology in mouse skeletal muscle fibers. Focus is given to the potency of this approach to investigate both fundamental aspects of excitation-contraction coupling and potential alterations of intracellular calcium handling in some muscle diseases.

Keywords: Skeletal muscle; voltage-clamp; calcium channel; voltage-sensor; intracellular calcium; SR calcium release; muscle dystrophy

Document Type: Miscellaneous

Publication date: 2004-04-01