Analysis of zinc in biological samples by flame atomic absorption spectrometry: Use of addition calibration technique

Authors: Dutra, Rosilene¹; Cantos, Geny²; Carasek, Eduardo¹

Source: Biological Trace Element Research, Volume 111, Numbers 1-3, June 2006 , pp. 265-279(15)

Buy & download fulltext article:

Price: $42.00 plus tax (Refund Policy)

Abstract:

The quantification of target analytes in complex matrices requires special calibration approaches to compensate for additional capacity or activity in the matrix samples. The standard addition is one of the most important calibration procedures for quantification of analytes in such matrices. However, this technique requires a great number of reagents and material, and its consumes a considerable amount of time throughout the analysis. In this work, a new calibration procedure to analyze biological samples is proposed. The proposed calibration, called the addition calibration technique, was used for the determination of zinc (Zn) in blood serum and erythrocyte samples. The results obtained were compared with those obtained using conventional calibration techniques (standard addition and standard calibration). The proposed addition calibration was validated by recovery tests using blood samples spiked with Zn. The range of recovery for blood serum and erythrocyte samples were 90-132% and 76-112%, respectively. Statistical studies among results obtained by the addition technique and conventional techniques, using a paired two-tailed Student's t-test and linear regression, demonstrated good agreement among them.

Keywords: Zinc; addition calibration technique; blood serum; erythrocyte; FAAS

Document Type: Research article

DOI: http://dx.doi.org/10.1385/BTER:111:1:265

Affiliations: 1: Departmento de Química, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil, 2: Departmento de Análises Clínicas, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil,

Publication date: 2006-06-01