Enhancement of rhamnoplipid production in residual soybean oil by an isolated strain of Pseudomonas aeruginosa
Source: Applied Biochemistry and Biotechnology, Volume 137, Number 1, December 2007 , pp. 463-470(8)
Publisher: Humana Press
Abstract:In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite expeirmental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 ± 1°C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH4NO3, and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively.
Document Type: Research article
Affiliations: 1: Universidade Federal de Uberlândia/FEQ, Caixa Postal 593, 38400-902, UberlândiaMG, Brazil, 2: Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21.949-900, Rio de JaneiroRJ, Brazil, 3: Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21.949-900, Rio de JaneiroRJ, Brazil, Email: email@example.com
Publication date: 2007-12-01