Enhancement of rhamnoplipid production in residual soybean oil by an isolated strain of Pseudomonas aeruginosa

Authors: Lima, C.¹; França, F.²; Sérvulo, E.³; Resende, M.¹; Cardoso, V.¹

Source: Applied Biochemistry and Biotechnology, Volume 137, Number 1, December 2007 , pp. 463-470(8)

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Abstract:

In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite expeirmental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 ± 1°C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH₄NO₃, and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively.

Keywords: Biosurfactant; experimental design; Pseudomonas aeruginosa; rhamnolipids; surface tension; soybean oil

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s12010-007-9072-0

Affiliations: 1: Universidade Federal de Uberlândia/FEQ, Caixa Postal 593, 38400-902, UberlândiaMG, Brazil, 2: Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21.949-900, Rio de JaneiroRJ, Brazil, 3: Escola de Química, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21.949-900, Rio de JaneiroRJ, Brazil, Email: eliana@eq.ufrj.br

Publication date: 2007-12-01