Novel core-shell nanoparticles and their application in high-capacity immobilization of enzymes

Authors: Mao, Xuepu¹; Huang, Jinfeng¹; Fai Leung, Man²; Du, Zhiyun¹; Ma, Lin³; Huang, Zhishu¹; Li, Pei⁴; Gu, Lianquan¹

Source: Applied Biochemistry and Biotechnology, Volume 135, Number 3, December 2006 , pp. 229-239(11)

Publisher: Humana Press

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Abstract:

Novel core-shell nanoparticles consisting of poly(methyl methacrylate) (PMMA) cores coated with synthetic polymer and biopolymer (polyethyleneimine, chitosan, and casein) shells were synthesized via direct graft copolymerization of methyl methacry late from hydrophilic polymers in the absence of surfactant. Average hydrodynamic diameters of the nanoparticles ranged from 163 to 263 nm. High-capacity (up to 530 mg/g) immobilizations of enzymes and high-activity retained percentage (E _spe) (up to 90%) were achieved.

Keywords: Core shell; poly(methyl methacrylate); nanoparticles; immobilized cellulase

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:135:3:229

Affiliations: 1: School of Pharmaceutical Sciences, Sun Yat-sen University, 510080, Guangzhou, P.R. China, 2: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China, 3: School of Chemistry and Chemical Engineering, Sun Yat-sen University, 510275, Guangzhou, P.R. China, 4: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China, Email: bcpeiLi@polyu.edu.hk

Publication date: 2006-12-01