Authors: Lee, Kwang-Min¹; Rhee, Chang-Hoon²; Kang, Choong-Kyung³; Kim, Jung-Hoe²

Source: Applied Biochemistry and Biotechnology, Volume 135, Number 1, October 2006 , pp. 81-100(20)

Publisher: Humana Press

Abstract:

The medium formulation and robust process modeling for anti-HIV peptide (T-20) production by recombinant Escherichia coli overexpression were studied by employing a crossed experimental design. The crossed design, a mixture design combined with process factor (induction duration), was used to find the optimal medium formulation and process time. The optimal settings for three major components, (7.75 mL of NPK sources, 5.5 mL of glucose, and 11.75 mL of MgSO₄) characterized by %T-20 (14.45%), the proportion of peptide to the total protein, were observed in a total of 100 mL of medium inducted at an optical density of 0.67 with 0.7 mM isopropyl-β-d-thiogalactopyranoside) for a 3-h induction duration at shake-flask scale. These conditions were further investigated to find robust, process conditions (8.2 mL of NPK sources, 5.6 mL of glucose, and 11.3 mL of MgSO₄, and a 3.5-h induction duration time) for T-20 production (13.9%) by applying propagation of error.

Keywords: Anti-HIV peptide; statistical formulation; crossed design; robust process; propagation of error

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:135:1:81

Affiliations: 1: Cellular Metabolic Engineering Lab., Korea Advanced Institute of Science and Technology, 737-1 Guseong-dong Yuseong-gu, 305-701, Daejeon, South Korea, Email: Klee0922@yahoo.co.kr 2: Cellular Metabolic Engineering Lab., Korea Advanced Institute of Science and Technology, 737-1 Guseong-dong Yuseong-gu, 305-701, Daejeon, South Korea, 3: KoBioTech Co., Ltd., 713-12 Gojan-dong Nam-gu, 405-821, Inchen, South Korea,

Publication date: 2006-10-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Anatomy & Physiology ,  Biotechnology ,  Organic Chemistry ,  Biochemistry
• By this author: Lee, Kwang-Min ;  Rhee, Chang-Hoon ;  Kang, Choong-Kyung ;  Kim, Jung-Hoe

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers