Authors: Mondragón-Parada, María¹; Nájera-Martínez, Minerva¹; Juárez-Ramírez, Cleotilde¹; Galíndez-Mayer, Juvencio¹; Ruiz-Ordaz, Nora¹; Cristiani-Urbina, Eliseo²

Source: Applied Biochemistry and Biotechnology, Volume 134, Number 3, September 2006 , pp. 223-232(10)

Publisher: Humana Press

Abstract:

The main purpose of this work was to isolate and characterize lactic acid bacteria (LAB) strains to be used for biomass production using a whey-based medium supplemented with an ammonium salt and with very low levels of yeast extract (0.25 g/L). Five strains of LAB were isolated from naturally soured milk after enrichment in whey-based medium. One bacterial isolate, designated MNM2, exhibited a remarkable capability to utilize whey lactose and give a high biomass yield on lactose. This strain was identified as Lactobacillus casei by its 16S rDNA sequence. A kinetic study of cell growth, lactose consumption, and titratable acidity production of this bacterial strain was performed in a bioreactor. The biomass yield on lactose, the percentage of lactose consumption, and the maximum increase in cell mass obtained in the bioreactor were 0.165 g of biomass/g of lactose, 100%, and 2.0 g/L, respectively, which were 1.44,1.11, and 2.35 times higher than those found in flask cultures. The results suggest that it is possible to produce LAB biomass from a whey-based medium supplemented with minimal amounts of yeast extract.

Keywords: Ammonium salts; biomass; lactic acid bacteria; whey; yeast extract

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:134:3:223

Affiliations: 1: Departamento de Ingeniería Bioquímica, Escuele Naciona de Ciencias Biológicas, del I.P.N. Carpio y Plan de Ayala, “Centro Operativo Naranjo”, Jaime Torres Bodet No. 142, Col Santa María la Rivera, Apdo, Postal C.O.N.-256, C.P. 06401, México, D.F., México, 2: Departamento de Ingeniería Bioquímica, Escuele Naciona de Ciencias Biológicas, del I.P.N. Carpio y Plan de Ayala, “Centro Operativo Naranjo”, Jaime Torres Bodet No. 142, Col Santa María la Rivera, Apdo, Postal C.O.N.-256, C.P. 06401, México, D.F., México, Email: ecristia@encb.ipn.mx

Publication date: 2006-09-01

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• In this Subject: Anatomy & Physiology ,  Biotechnology ,  Organic Chemistry ,  Biochemistry
• By this author: Mondragón-Parada, María ;  Nájera-Martínez, Minerva ;  Juárez-Ramírez, Cleotilde ;  Galíndez-Mayer, Juvencio ;  Ruiz-Ordaz, Nora ;  Cristiani-Urbina, Eliseo

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