Methyl jasmonate stimulates jaceosidin and hispidulin production in cell cultures of Saussurea medusa

Authors: Fu, Chun-Xiang¹; Cheng, Li-Qin¹; Lv, Xiao-Fen¹; Zhao, De-Xiu²; Ma, Fengshan³

Source: Applied Biochemistry and Biotechnology, Volume 134, Number 1, July 2006 , pp. 89-96(8)

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Abstract:

Cell cultures of Saussurea medusa produce valuable secondary metabolites, and jaceosidin and hispidulin are the major bioactive compounds. In the present study, the cultures were challenged by methyl jasmonate (MJ). The highest jaceosidin and hispidulin concentrations (65.2±3.67 mg/L and 12.3±0.47 mg/L) were achieved with 5 μM MJ added to 9-d-old subcultures, being 2.2-fold and 4.2-fold, respectively, higher than those from controls. The elicitor had little influence on cell growth, indicating that the changed biological processes did not include alterations in cell division. Furthermore, we observed that the activities of phenylalanine ammonia lyase were transiently increased after treatment with MJ, which suggests that this elicitor modifies jaceosidin and hispidulin production by regulating the phenylpropanoid pathway.

Keywords: Jaceosidin; hispidulin; methyl jasmonate; phenylalanine ammonia lyase; Saussurea medusa

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:134:1:89

Affiliations: 1: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China, 2: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China, Email: zhaodx@ibcas.ac.cn 3: Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada,

Publication date: 2006-07-01