Effect of copper and manganese ions on activities of laccase and peroxidases in three Pleurotus species grown on agricultural wastes

Authors: Stajić, Mirjana¹; Persky, Limor²; Hadar, Yitzhak²; Friesem, Dana²; Duletić-Laušević, Sonja³; Wasser, Solomon⁴; Nevo, Eviatar⁴

Source: Applied Biochemistry and Biotechnology, Volume 128, Number 1, January 2006 , pp. 87-96(10)

Publisher: Humana Press

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Abstract:

Copper (Cu²⁺) and manganese (Mn²⁺) ions influenced laccase (Lac) and peroxidase production in Pleurotus eryngii, Pleurotus ostreatus, and Pleurotus pulmonarius. In P. eryngii, the optimum Cu²⁺ concentration for Lac production was 1 mM and for peroxidases 10mM, and Mn²⁺ concentration of 5mM led to peaks of Lac and peroxidase activity. In P. ostreatus HAI 493, the highest level of Lac activity was at Cu²⁺ concentrations of 1 and 10 mM and Mn²⁺ concentration of 1mM, respectively. The absence of Cu²⁺ and Mn²⁺ caused the highest levels of peroxidase production. In P. ostreatus HAI 494, the highest level of Lac activity was at a Cu²⁺ concentration of 5 mM and at Mn²⁺ concentration of 1 mM, respectively. High levels of peroxidase activity were found in the medium without and with 1mM Cu²⁺, and at 1 and 5 mM Mn²⁺, respectively. In P. pulmonarius, the highest Lac activity was found in the presence of 5 mM Cu²⁺ and 5 mM Mn²⁺, respectively. The absence of Cu²⁺ and Mn²⁺ as well as their presence at a concentration of 1 mM led to the peaks of peroxidase activities.

Keywords: Pleurotus eryngii; Pleurotus ostreatus; Pleurotus pulmonarius; laccase; peroxidases; copper; manganese

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:128:1:087

Affiliations: 1: Institute of Botany, Faculty of Biology, University of Belgrade, Takovska 43, 11000, Belgrade, Serbia and Montenegro, Email: stajicm@bfbot.bg.ac.yu 2: Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, PO Box 12, 76100, Rehovot, Israel, 3: Institute of Botany, Faculty of Biology, University of Belgrade, Takovska 43, 11000, Belgrade, Serbia and Montenegro, 4: Institute of Evolution, University of Haifa, Mount Carmel, 31905, Haifa, Israel,

Publication date: 2006-01-01

Effect of copper and manganese ions on activities of laccase and peroxidases in three Pleurotus species grown on agricultural wastes

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