Authors: Yu, Huijun¹; Liu, Junqui²; Li, Jing¹; Zang, Tianzhu³; Luo, Guimin³; Shen, Jiacong¹

Source: Applied Biochemistry and Biotechnology, Volume 127, Number 2, November 2005 , pp. 133-142(10)

Publisher: Humana Press

Abstract:

The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochrome-c oxidase activity were selected to evaluate the preservation of mitochondrial integrity in this system. Seleno-LuGST1-1 could effectively protect the mitochondria against oxidative damage in a dose-dependent manner and exhibited both higher catalytic activity and greater antioxidant ability than the classic mimic, 2-phenyl-1,2-benziososelenazol-3(2H)-one (Ebselen). This novel artificial biocatalyst therefore may have great protential for pharmacologic application in the treatment of reactive oxygen species-related diseases.

Keywords: Antioxidant activity; artificial enzymes; mitochondria; oxidative stress; glutathione peroxidase; selenium-containing glutathione transferase

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:127:2:133

Affiliations: 1: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, 130012, Changchun, People's Republic of China, 2: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, 130012, Changchun, People's Republic of China, Email: jqliu2000@yahoo.com 3: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, People's Republic of China,

Publication date: 2005-11-01

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• In this Subject: Anatomy & Physiology ,  Biotechnology ,  Organic Chemistry ,  Biochemistry
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