Protection of mitochondrial integrity from oxidative stress by selenium-containing glutathione transferase
Source: Applied Biochemistry and Biotechnology, Volume 127, Number 2, November 2005 , pp. 133-142(10)
Publisher: Humana Press
Abstract:The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochrome-c oxidase activity were selected to evaluate the preservation of mitochondrial integrity in this system. Seleno-LuGST1-1 could effectively protect the mitochondria against oxidative damage in a dose-dependent manner and exhibited both higher catalytic activity and greater antioxidant ability than the classic mimic, 2-phenyl-1,2-benziososelenazol-3(2H)-one (Ebselen). This novel artificial biocatalyst therefore may have great protential for pharmacologic application in the treatment of reactive oxygen species-related diseases.
Document Type: Research Article
Affiliations: 1: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, 130012, Changchun, People's Republic of China, 2: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, 130012, Changchun, People's Republic of China, Email: email@example.com 3: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, People's Republic of China,
Publication date: November 1, 2005