Effect of hydrocarbons and other parameters on hydrocarbon-utilizing Pichia angusta MTCC-225

Authors: Sreenivas Rao, R.¹; Rasheed, M.²; Kalpana, G.²; Patil, D.²; Kumar, B.²

Source: Applied Biochemistry and Biotechnology, Volume 126, Number 3, September 2005 , pp. 205-214(10)

Buy & download fulltext article:

Price: $42.00 plus tax (Refund Policy)

Abstract:

Pichia angusta MTCC-225, a catalase-positive yeast that utilizes methanol and lighter hydrocarbons, is the subject of this investigation. An orthogonal experimental design L₁₆ was used to investigate the effects of methanol, a gas mixture, zero air, temperature, agitation, and salts solution on hydrocarbon utilizing P. angusta. QUALITEK-4 Software was used for automatic design and analysis of the experimental results. Among the various parameters tested, agitation contributed the highest influence (56.5%). Zero air, methanol concentration, and gas mixture showed a moderate influence on the growth of P. angusta. Methanol concentration and gas mixture showed a 10.91 and 10.12% influence, respectively, on yeast growth. Zero air played an important role, with a 15.19% influence on the utilization of hydrocarbon.

Keywords: Hydrocarbons; methanol; orthogonal array; Pichia angusta

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:126:3:205

Affiliations: 1: National Geophysical Research Institute, Uppal Road, 500007, Hyderabad, India, Email: srinu_ravella@yahoo.com 2: National Geophysical Research Institute, Uppal Road, 500007, Hyderabad, India,

Publication date: 2005-09-01