Effect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawater

Authors: Kong, Bo¹; Huang, He-Qing²; Lin, Qing-Mei³; Cai, Zong-Wei⁴; Chen, Ping¹

Source: Applied Biochemistry and Biotechnology, Volume 126, Number 2, August 2005 , pp. 133-148(16)

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Abstract:

We describe a protein reactor consisting of native liver ferritin of Dasyatis akajei (DALF) and a dialysis bag. Our goal was to study a ferritin reactor for its capacity to trap various heavy metal ions (M²⁺) in flowing seawater. The reactor is sensitive and inexpensive and can be operated by nonprofessional technicians. A positive relationship between the number of trapped M²⁺ with the DALF reactor and its concentration in the flowing seawater was observed. Both the pH in the medium and the phosphate content within the ferritin cavity strongly affected trapping capacity. It was found that the ferritin released its phosphate compound directly with a shift in pH without the need for releasing reagent, which differs from the phosphate release characteristics of horse spleen ferritin, as previously described. This behavior evidently makes the trapping capacity with the ferritin reactor weaken, indicating that this trapping capacity is tightly connected to its phosphate compound. Our study shows that a self-regulation ability of the ferritin shell rather than its phosphate compound plays an important role in controlling the rate and capacity of trapping M²⁺. The ferritin reactor was constructed to monitor the contamination level of M²⁺ in flowing seawater. Our preliminary data along with fieldwork indicate that the DALF reactor is an analytical means for effectively monitoring the contamination level of M²⁺ in flowing seawater.

Keywords: Dasyatis akajei; heavy metalions; ferritin reactor; phosphate effect; seawater; contamination level

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:126:2:133

Affiliations: 1: Department of Biochemistry and Biotechnology, School of Life Sciences, State Key Laboratory of Marine Environmental Science, Xiamen University, 361005, Xiamen, China, 2: Department of Biochemistry and Biotechnology, School of Life Sciences, State Key Laboratory of Marine Environmental Science, Xiamen University, 361005, Xiamen, China, Email: hqhuang@jingxian.xme.edu.cn 3: The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, 361005, Xiamen, China, 4: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong,

Publication date: 2005-08-01

Effect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawater

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