Simultaneous biocatalyst production and baeyer-villiger oxidation for bioonversion of cyclohexanone by recombinant Escherichia coli expressing cyclohexanone monooxygenase

Authors: Lee, Won-Heong¹; Park, Yong-Cheol¹; Lee, Dae-Hee¹; Park, Kyungmoon²; Seo, Jin-Ho³

Source: Applied Biochemistry and Biotechnology, Volume 123, Numbers 1-3, March 2005 , pp. 827-836(10)

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Abstract:

Cyclohexanone monooxygenase (CHMO) catalyzing Baeyer-Villiger oxidation converts cyclic ketones into optically pure lactones, which have been used as building blocks in organic synthesis. A recombinant Escherichia coli BL21(DE3)/pMM4 expressing CHMO originated from Acinetobacter sp. NCIB 9871 was used to produce ε-caprolactone through a simultaneous biocatalyst production and Baeyer-Villiger oxidation (SPO) process. Afed-batch process was designed to obtain high cell density for improvin production of ε-caprolactone. The fed-batch SPO process have the best results, 10.2 g/L of ε-caprolactone and 0.34 g/(L·h) of productivity, corresponding to a 10.5- and 3.4-fold enhancement compared with those of the batch SPO, respectively.

Keywords: Simultaneous biocatalyst production and Baeyer-Villiger oxidation; cyclohexanone monooxygenase; Escherichia coli; fed-batch process; ε-caprolactone

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:123:1-3:0827

Affiliations: 1: School of Agricultural Biotechnology, Seoul National University, 151-742, Seoul, Korea, 2: Department of Chemical System Engineering, Hongik University, Yeongigun, 339-701, Chungnam, Korea, 3: School of Agricultural Biotechnology, Seoul National University, 151-742, Seoul, Korea, Email: jhseo94@snu.ac.kr

Publication date: 2005-03-01