Home >> Applied Biochemistry and Biotechnology, Volume 123, Numbers 1-3

A glycoside flavonoid in kudzu (Pueraria lobata): Identification, quantification, and determination of antioxidant activity

Authors: Lau, Ching1; Carrier, Danielle2; Beitle, Robert1; Howard, Luke3; Lay, Jackson4; Liyanage, Rohana4; Clausen, Edgar1

Source: Applied Biochemistry and Biotechnology, Volume 123, Numbers 1-3, March 2005 , pp. 783-794(12)

Publisher: Humana Press

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Abstract:

Kudzu (Pueraria lobata) foliage has been touted as a possible energy crop. High-performance liquid chromatography and mass spectrometry analysis of the methanolic kudzu foliage extracts confirmed the presence of robinin (kaempferol-3-O-robinoside-7-O-rhamnoside). Robinin accounted for 0.65±0.16% (dry basis) of kudzu biomass. Fast performance liquid chromatography (FPLC) was employed to fractionate robinin from the crude extract. The antioxidant capacity of robinin was evaluated by an oxygen radical absorbance capacity (ORAC) assay. The ORAC values of pure standard were compared with those of the extract fractions. One milligram of the FPLC-fractionated robinin generated an ORAC value of 5.15±2.00 μmol/mg of Trolox, whereas 1 mg of pure robinin generated an ORAC value of 12.34±0.45 μmol/mg of Trolox. Because of its antioxidant properties, robinin may be a flavonoid worth extracting prior to energy production.

Keywords: Pueraria lobata; high-performance liquid chromatography; oxygen radical absorbance capacity; antioxidant; robinin; flavonoids

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:123:1-3:0783

Affiliations: 1: Department of Chemical Engineering, University of Arkansas, 3202 Bell Engineering Center, 72701, Fayetteville, AR, 2: Department of Biological and Agricultural Engineering, University of Arkansas, 203 Engineering Hall, 72701, Fayetteville, AR, Email: carrier@uark.edu 3: Department of Food Science, University of Arkansas, 2650 N. Young Avenue, 72701, Fayetteville, AR, 4: Department of Chemistry and Biochemistry, University of Arkansas, 115 Chemistry Building, 72701, Fayetteville, AR,

Publication date: 2005-03-01

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