Effects of sugar inhibition on cellulases and β-glucosidase during enzymatic hydrolysis of softwood substrates
Authors: Xiao, Zhizhuang1; Zhang, Xiao1; Gregg, David1; Saddler, John2
Source: Applied Biochemistry and Biotechnology, Volume 115, Numbers 1-3, March 2004 , pp. 1115-1126(12)
Publisher: Humana Press
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Abstract:
A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and β-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both β-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on β-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.Keywords: β-Glucosidase; cellulase; degree of inhibition; softwood; glucose; hydrolysate
Document Type: Research article
DOI: 10.1385/ABAB:115:1-3:1115
Affiliations: 1: Forest Products Biotechnology, University of British Columbia, 4033-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada, 2: Forest Products Biotechnology, University of British Columbia, 4033-2424 Main Mall, V6T 1Z4, Vancouver, BC, Canada, Email: saddler@interchg.ubc.ca
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