Comparison of microbial inhibition and enzymatic hydrolysis rates of liquid and solid fractions produced from pretreatment of biomass with carbonic acid and liquid hot water

Authors: Yourchisin, Damo¹; Peter Van Walsum, G.²

Source: Applied Biochemistry and Biotechnology, Volume 115, Numbers 1-3, March 2004 , pp. 1073-1086(14)

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Abstract:

This research quantified the enzymatic digestibility of the solid component and the microbial inhibition of the liquid component of pretreated aspen wood and cornstover hydrolysates. Products of liquid hot water and carbonic acid pretreatment were compared. Pretreatment temperatures tested ranged from 180 to 220°C, and reaction times were varied between 4 and 64 min. Both microbial inhibition rates and enzymatic hydrolysis rates showed no difference between pretreatments containing carbonic acid and those not containing no carbonic acid. Microbial inhibition increased as the reaction severity increased, but only above a midpoint severity parameter of 200°C for 16 min. Both the rates and yields of enzymatic hydrolysis displayed an increase from the lowest tested reaction severity to the highest tested reaction severity.

Keywords: Carbonic acid; pretreatment; hydrolysis; corn stover; aspen wood

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:115:1-3:1073

Affiliations: 1: Chemical Division (Concepts), US Army Maneuver Support Center (MANSCEN), Directorate of Combat Developments, 320 Manscen Loop, Suite 141, 65473-8929, Fort Leonard Wood, MO, 2: Department of Environmental Studies and Glasscock Energy Research Center, Baylor University, PO Box 97266, 76798-7266, Waco, TX, Email: GPeter_van_Walsum@Baylor.edu

Publication date: 2004-03-01