Effect of kLa on the Production of Glucose 6-Phosphate Dehydrogenase from Saccharomyces cerevisiae Grown by Fermentation Process
Authors: Pereira Silva, D.; Pessoa Jr., A.; Roberto, I.C.; Vitolo, M.
Source: Applied Biochemistry and Biotechnology, Volume 98, Numbers 1-3, March 2002 , pp. 205-214(10)
Publisher: Humana Press
Abstract:In a 5-L fermentor (NBS-MF 105), Saccharomyces cerevisiae (0.7 g/L) was inoculated into a liquid medium (pH 4.0) containing 17 g/L of glucose, 2.55 g/L of yeast extract, 4.25 g/L of peptone, 2.04 g/L of Na2HPO4·12H2O, 4.34 g/L of (NH4)2SO4 and 0.064 g/L of MgSO4·7H2O and aerobically cultivated at 35°C for 22 h. Agitation and aeration were adjusted to attain initial kLa values of 15, 60, 135, and 230 h-1. The glucose 6-phosphate dehydrogenase (G6PDH) productivity (PrG6PDH) obtained for kLa values of 15, 60, 135, and 230 h-1 was 10.6, 31.8, 30.3, and 23.3 U/([Láh]), respectively, whereas the cell productivity (PrX) for the same kLavalues were 0.24, 0.69, 0.69, and 0.49 g/[Láh], respectively. Thus, both events are coupled and depend on the dissolved oxygen in the medium.
Document Type: Research Article
Publication date: 2002-03-01