Isolation of Magnetospirillum magneticum AMB-1 Mutants Defective in Bacterial Magnetic Particle Synthesis by Transposon Mutagenesis
Authors: Matsunaga, T.; Wahyudi, A.T.; Takeyama, H.
Source: Applied Biochemistry and Biotechnology, Volume 91, Numbers 1-3, March 2001 , pp. 147-154(8)
Publisher: Humana Press
Abstract:Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10-7per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.
Document Type: Research Article
Publication date: March 1, 2001