Studies on alkaline protease produced by Bacillus sp. NG312

Authors: S., Jasvir¹; Gill, Navdeep¹; Devasahayam, Gina¹; Sahoo, Debendra²

Source: Applied Biochemistry and Biotechnology, Volume 76, Number 1, February 1999 , pp. 57-63(7)

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Abstract:

An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at pH 11.0 and 60°C. The temperature optimum was increased by 10°C in presence of Ca²⁺. The crudeenzyme was found to have half-life of 11 d at 37°C and maximum stability at pH 9.0-10.0. It also exhibited very good stability in presence of detergent components and some locally available commericial detergent powders.

Keywords: Alkaline protease; Bacillus; characterization; detergent compatibility

Document Type: Research article

DOI: http://dx.doi.org/10.1385/ABAB:76:1:57

Affiliations: 1: Institute of Microbial Technology, Sector 39-A, 160 036, Chandigarh, India, 2: Institute of Microbial Technology, Sector 39-A, 160 036, Chandigarh, India, Email: debsahoo@mit.edu

Publication date: 1999-02-01