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Open Access Poria cocos water extract (PCW) protects PC1 2 neuronal cells from beta-amyloid-induced cell death through antioxidant and antiapoptotic functions

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Beta-amyloid (Abeta)-induced neurotoxicity is considered to be mediated through the formation of reactive oxygen species (ROS). In this study, the protective effects of Poria cocos water extract (PCW) against Abeta1-42-induced cell death were investigated using rat pheochromocytoma (PC12) cells. Exposure of PC12 cells to the Abeta1-42 (20 μM) for 48h resulted in neuronal cell death, whereas pretreatment with PCW at the concentration range of 5-125 μg/ml reduced Abeta1-42-induced cell death. In addition, PC12 cells treated with Abeta1-42 exhibited increased accumulation of intracellular oxidative damages and underwent apoptotic death as determined by characteristic morphological alterations and positive in situ terminal end-labeling (TUNEL staining). However, PCW attenuated Abeta1-42-induced cytotoxicity, apoptotic features, and accumulation of intracellular oxidative damage. Moreover, PCW (5 to 125 μg/ml) decreased expression of apoptotic protein Bax and activity of caspase-3, but enhanced expression of anti-apoptotic protein Bcl-2. These results suggest that PCW may protect cells through suppressing the oxidative stress and the apoptosis induced by Abeta1-42, implying that PCW may be potential natural agents for Alzheimer's diseases.
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Document Type: Research Article

Affiliations: 1: INAM Neuroscience Research Center, Wonkwang University, Gunpo-Si, Gyunggi-Do, South Korea 2: Department of Neurology, Wonkwang University, Gunpo-Si, Gyunggi-Do, South Korea 3: College of Oriental Medicine, Wonkwang University, Department of Oriental Neuropsychiatry, Wonkwang University, Gunpo-Si, Gyunggi-Do, South Korea 4: College of Oriental Medicine, Dongeui University, Department of Neuropsychiaty, College of Oriental Medicine, Wonkwang University, Gunpo-Si, Gyunggi-Do, South Korea 5: INAM Neuroscience Research Center, Wonkwang University, Gunpo-Si, Gyunggi-Do, 435-040, South Korea, Email: [email protected] 6: Sanbon Medical Center, Department of Oriental Neuropsychiatry Medicine, Wonkwang University, Gunpo-Si, Gyunggi-Do, 435-040, South Korea, Email: [email protected]

Publication date: 2009-11-01

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