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Suitability of Smopex®-102 cation-exchange fiber for analytical purposes and drug monitoring

Authors: Karppi, J.1; Karppi, J.2; Åkerman, S.3; Åkerman, K.4; Kontturi, K.5; Nyyssönen, K.6; Penttilä, I.1; Penttilä, I.7

Source: Die Pharmazie - An International Journal of Pharmaceutical Sciences, Volume 64, Number 1, 1 January 2009 , pp. 14-18(5)

Publisher: Govi-Verlag

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Abstract:

The present study aimed to evaluate the suitability of Smopex®-102 cation-exchange fiber for the separation of acidic and basic model drugs from biological fluids (e.g. serum) prior to chromatographic analysis. In addition, the interactions of the drugs with the fiber were studied. The study found that basic antidepressant model drugs bound to a considerably greater extent than acidic drugs to poly(acrylic acid) (PAA) grafted Smopex®-102 cation-exchange fiber from 25 mM HEPES buffer (pH 7.0) and spiked serum. Drug binding from serum decreased except for acidic drugs due to drug distribution between serum proteins and cation-exchange fiber. Electrostatic interactions were possibly the most important factors affecting drug binding to the fiber. Basic drugs were released most effectively from the fiber by using acetic acid (mean released amount 123.7 ± 36.3% and mean absolute recovery 95.4 ± 23.8%). Results demonstrated that the cation-exchange fiber evaluated might be a potential material for separating basic drugs from protein–free and proteinaceous (e.g. serum) liquid solutions for subsequent monitoring and evaluation. However, the drug release solution and release time must be optimized more precisely in order to validate described sample preparation method for each basic drug.

Document Type: Research Article

DOI: http://dx.doi.org/10.1691/ph.2008.8153

Affiliations: 1: Laboratory Centre, Kuopio University Hospital, Kuopio, Finland 2: Research Institute of Public Health, School of Public Health and Clinical Nutrition, University of Kuopio, P.O. Box 1627, Kuopio, FI-70211, Finland, Email: jouni.karppi@uku.fi 3: Kajaanin I apteekki, Kajaani, Finland 4: Department of Clinical Chemistry, EP Central Hospital, Seinäjoki, Finland 5: Laboratory of Physical Chemistry and Electrochemistry, Helsinki University of Technology, Hut, Finland 6: Research Institute of Public Health, School of Public Health and Clinical Nutrition, University of Kuopio, Kuopio, Finland 7: Kuopio Research Institute of Exercise Medicine, Kuopio, Finland

Publication date: January 1, 2009

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  • Pharmazie is one of the world's leading pharmaceutical journals. As a peer-reviewed scientific journal, DiePharmazie is regularly indexed in Current Contents/Life Sciences, Excerpta Medica, Analytical Abstracts, International Pharmaceutical Abstracts, Beilstein Current Facts in Chemistry, Chemical Engineering and Biotechnology Abstracts (CEABA) and Science Citation Index.
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