@article {Cappra Silva:2007:0031-7144:732,
title = "Development and validation of a LC method for determination of genistein in topical nanoemulsions",
journal = "Die Pharmazie - An International Journal of Pharmaceutical Sciences",
parent_itemid = "infobike://govi/pharmaz",
publishercode ="govi",
year = "2007",
volume = "62",
number = "10",
publication date ="2007-10-01T00:00:00",
pages = "732-734",
itemtype = "ARTICLE",
issn = "0031-7144",
url = "https://www.ingentaconnect.com/content/govi/pharmaz/2007/00000062/00000010/art00003",
doi = "doi:10.1691/ph.2007.10.7044",
author = "Cappra Silva, A. P. and Koester, L. S. and Mayorga, P. and Linck Bassani, V. and Teixeira, H.",
abstract = "The aim of this study was to develop and validate an isocratic LC method for the quantification of genistein in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water/acetonitrile
(70 : 25 : 5, w/w/w) at 1.0 ml \textperiodcentered min1. The detection was carried out on a UV detector at 327 nm. The linearity, in the range of 2575 g/ml, presented a determination coefficient (r2) higher than 0.999, calculated by the least square method. No
interferences from the excipients (egg-lecithin, octyldodecanol or medium chain triglycerides) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2.3%. The recovery of genistein from nanoemulsions ranged from 96.6% to 106.6%.
The excellent performance of the method, its linearity, accuracy and precision, demonstrate that it can be readily used to quantify genistein incorporated in nanoemulsions.",
}