Development and validation of a LC method for determination of genistein in topical nanoemulsions
Abstract:The aim of this study was to develop and validate an isocratic LC method for the quantification of genistein in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water/acetonitrile (70 : 25 : 5, w/w/w) at 1.0 ml · min–1. The detection was carried out on a UV detector at 327 nm. The linearity, in the range of 25–75 μg/ml, presented a determination coefficient (r2) higher than 0.999, calculated by the least square method. No interferences from the excipients (egg-lecithin, octyldodecanol or medium chain triglycerides) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2.3%. The recovery of genistein from nanoemulsions ranged from 96.6% to 106.6%. The excellent performance of the method, its linearity, accuracy and precision, demonstrate that it can be readily used to quantify genistein incorporated in nanoemulsions.
Document Type: Research Article
Affiliations: 1: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 2: Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752/404A, Porto Alegre, RS, 90610-000, Brazil, Email: email@example.com
Publication date: 2007-10-01
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