Skip to main content

Open Access Development and validation of a LC method for determination of genistein in topical nanoemulsions

Download Article:
 Download
(PDF 78.4 kb)
 

Abstract:

The aim of this study was to develop and validate an isocratic LC method for the quantification of genistein in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water/acetonitrile (70 : 25 : 5, w/w/w) at 1.0 ml ยท min–1. The detection was carried out on a UV detector at 327 nm. The linearity, in the range of 25–75 μg/ml, presented a determination coefficient (r2) higher than 0.999, calculated by the least square method. No interferences from the excipients (egg-lecithin, octyldodecanol or medium chain triglycerides) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2.3%. The recovery of genistein from nanoemulsions ranged from 96.6% to 106.6%. The excellent performance of the method, its linearity, accuracy and precision, demonstrate that it can be readily used to quantify genistein incorporated in nanoemulsions.

Document Type: Research Article

DOI: http://dx.doi.org/10.1691/ph.2007.10.7044

Affiliations: 1: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 2: Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752/404A, Porto Alegre, RS, 90610-000, Brazil, Email: helder@farmacia.ufrgs.br

Publication date: October 1, 2007

More about this publication?
  • Pharmazie is one of the world's leading pharmaceutical journals. As a peer-reviewed scientific journal, DiePharmazie is regularly indexed in Current Contents/Life Sciences, Excerpta Medica, Analytical Abstracts, International Pharmaceutical Abstracts, Beilstein Current Facts in Chemistry, Chemical Engineering and Biotechnology Abstracts (CEABA) and Science Citation Index.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
govi/pharmaz/2007/00000062/00000010/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more