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Open Access Development and validation of a LC method for determination of genistein in topical nanoemulsions

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The aim of this study was to develop and validate an isocratic LC method for the quantification of genistein in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water/acetonitrile (70 : 25 : 5, w/w/w) at 1.0 ml ยท min–1. The detection was carried out on a UV detector at 327 nm. The linearity, in the range of 25–75 μg/ml, presented a determination coefficient (r2) higher than 0.999, calculated by the least square method. No interferences from the excipients (egg-lecithin, octyldodecanol or medium chain triglycerides) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2.3%. The recovery of genistein from nanoemulsions ranged from 96.6% to 106.6%. The excellent performance of the method, its linearity, accuracy and precision, demonstrate that it can be readily used to quantify genistein incorporated in nanoemulsions.
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Document Type: Research Article

Affiliations: 1: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 2: Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752/404A, Porto Alegre, RS, 90610-000, Brazil, Email: [email protected]

Publication date: 01 October 2007

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  • Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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