Direct spectrophotometric determination of diacerhein in capsules
A simple, rapid and precise ultraviolet spectrophotometric method using 0.1 N sodium hydroxide has been developed and validated for the assay of diacerhein. The drug can be estimated at 277 nm (ultraviolet region, UV) as well as at 502 nm (visible region, VIS). The absorbances were
linearly correlated with concentration in the 6.0–24 μg · mL–1 range (r = 0.9999) and 10–34 μg · mL–1 range (r = 0.9998), respectively at 277 and at 502 nm. The relative standard deviation values for intra (n =
6) and inter-day (n = 3) precision were <2%. Recoveries ranged between 99.0 and 101.7%. The method has been successfully applied to the drug assay in capsules. It was also found that the excipients in the commercial capsules did not interfere with the method. Statistical
analysis showed no significant difference between the results obtained at two wavelengths (277 nm or 502 nm).
Document Type: Research Article
Affiliations: 1: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil 2: Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Strasse, Santa Maria, RS, CEP 97.105-900, Brazil, Email: [email protected]
Publication date: 01 July 2007
- Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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