Open Access Oxidative stress in Graves' disease patients and antioxidant protection against lymphocytes DNA damage in vitro

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Abstract:

DNA damage to peripheral blood lymphocytes of patients with Graves' disease (GD) was studied in vitro before and after treatment with antioxidants, melatonin, quercetin, N-acetylcysteine (NAC) and vitamin C. DNA damage (comet %) was remarkably higher in patients (23.7 ± 5.5%) than that in healthy persons (9.8 ± 3.2%, p < 0.01). Plasma malondialdehyde (MDA) content (7.90 ± 1.77 μM) of patients was significantly higher than that of healthy persons (4.71 ± 1.19 μM, p < 0.01). Also, the plasma total antioxidant capacity (TAC) (7.53 ± 1.35 U/ml) in GD patients was significantly lower than that in healthy persons (10.56 ± 2.21 U/ml, p < 0.01). Negative correlations were observed between plasma TAC and DNA damage in lymphocytes (r = –0.599, p < 0.01), and between plasma TAC and MDA (r = –0.40, p < 0.05) in GD patients. After treatment with 100 μM melatonin, quercetin or NAC for 4 h in vitro, DNA damage in lymphocytes in GD patients declined significantly (from 23.8 ± 4.4% to 14.4 ± 4.0%, p < 0.001 for melatonin, from 23.4 ± 4.7% to 18.1 ± 4.3%, p < 0.01 for quercetin, from 23.7 ± 4.0% to 18.7 ± 5.7%, p < 0.05 for NAC), while there was little change with concentrations of 1–100 μM of vitamin C. However, 1000 μM vitamin C enhanced DNA damage significantly (from 23.8 ± 2.3% to 30.3 ± 3.9%, p < 0.05). Our results showed that oxidative stress existed in GD patients and the antioxidants melatonin, quercetin and NAC are beneficial for DNA damage in lymphocytes of GD patients in vitro.

Document Type: Research Article

Affiliations: 1: School of Life Sciences, Lanzhou University, Lanzhou, China 2: The First Affiliated Hospital of Lanzhou Medical College, Lanzhou, China 3: Medical College of Northwest Minorities University, Lanzhou, China 4: School of Life Sciences, Lanzhou University, Lanzhou, 730000, China, Email: zhengrl@lzu.edu.cn

Publication date: September 1, 2005

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  • Pharmazie is one of the world's leading pharmaceutical journals. As a peer-reviewed scientific journal, DiePharmazie is regularly indexed in Current Contents/Life Sciences, Excerpta Medica, Analytical Abstracts, International Pharmaceutical Abstracts, Beilstein Current Facts in Chemistry, Chemical Engineering and Biotechnology Abstracts (CEABA) and Science Citation Index.
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