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Open Access Analysis of luteolin in Elsholtzia blanda Benth. by RP-HPLC

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The total luteolin content in Elsholtzia blanda Benth. extracts (EBBE) was determined using reversed phase HPLC. C18 was used as the packing material and 0.01 M phosphate buffer (pH 2)-tetrahydrofuran-isopropanol (70 : 30 : 5) as the mobile phase with detection wavelength 360 nm. The recovery of the method was 96.4% ∼ 101.8%, and the assay was linear at concentrations from 5 to 200 μg/ml (r = 0.9999). The results indicated that the content of luteolin in EBBE extracted under different conditions varied significantly. This method can be used to optimize the extraction procedure and determine the content of luteolin in Elsholtzia blanda Benth. extracts.
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Document Type: Research Article

Affiliations: 1: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China 2: Obstetric and Gynecology Hospital, Zhejiang University, Hangzhou, P.R. China 3: College of Pharmaceutical Sciences, Zhejiang University, 353 Yan'an Road, Hangzhou, 310031, P.R. China, Email: [email protected]

Publication date: 2005-09-01

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  • Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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