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Open Access Analysis of luteolin in Elsholtzia blanda Benth. by RP-HPLC

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The total luteolin content in Elsholtzia blanda Benth. extracts (EBBE) was determined using reversed phase HPLC. C18 was used as the packing material and 0.01 M phosphate buffer (pH 2)-tetrahydrofuran-isopropanol (70 : 30 : 5) as the mobile phase with detection wavelength 360 nm. The recovery of the method was 96.4% ∼ 101.8%, and the assay was linear at concentrations from 5 to 200 μg/ml (r = 0.9999). The results indicated that the content of luteolin in EBBE extracted under different conditions varied significantly. This method can be used to optimize the extraction procedure and determine the content of luteolin in Elsholtzia blanda Benth. extracts.

Document Type: Research Article

Affiliations: 1: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P.R. China 2: Obstetric and Gynecology Hospital, Zhejiang University, Hangzhou, P.R. China 3: College of Pharmaceutical Sciences, Zhejiang University, 353 Yan'an Road, Hangzhou, 310031, P.R. China, Email:

Publication date: September 1, 2005

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  • Pharmazie is one of the world's leading pharmaceutical journals. As a peer-reviewed scientific journal, DiePharmazie is regularly indexed in Current Contents/Life Sciences, Excerpta Medica, Analytical Abstracts, International Pharmaceutical Abstracts, Beilstein Current Facts in Chemistry, Chemical Engineering and Biotechnology Abstracts (CEABA) and Science Citation Index.
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