A series of hitherto unknown enantiopure (–)-ferruginine analogues of type 8 and 9 was prepared and tested for the affinity toward the nicotinic acetylcholine receptor (nAChR) subtypes (α4)2(β2)3, α7*, α3β4*,
and (α1)2β1γδ. The stereoconservative asymmetric syntheses started with (–)-cocainhydrochloride (10) from the chiral pool which was transformed into the chiral building blocks (+)-2-tropanone (11) and to (–)-anhydroecgonine (18).
Key steps of the syntheses are novel extensions to existing methodology e.g. a Suzuki Pd(0)-mediated cross-coupling of vinyl triflate (12) with the heteroaryl organoboranes 13–15 and an inverse type [4+2]-cycloaddition with 1,2,4,5-tetrazine (21). The
bioisosteric replacement of the 3-acetyl pharmacophoric element of the lead 6 by a 3-pyridyl, 5-chloropyridyl, 5-pyrimidinyl, 2-pyrazinyl, or 4-pyridazinyl moiety resulted in nAChR ligands with Ki-values ranging from 1.1–713 nM toward the (α4)2(β2)3
subtype combined with significant differentiation among the nAChR subtypes when tested in vitro by radioligand binding studies. Generally the ferrugininoids are less potent than the corresponding norferrugininoids. Similar to results of the norferrugininoid series the novel azine substituted
ferrugininoids 8 proved to be more potent than the diazine analogues 9; both exhibited higher affinities compared to the lead 6. The 5-chloropyridyl-containing variant 8b [1R, 5S)-enantiomer] turned out to be the most active nAChR ligand with a 12-fold
higher affinity toward the (α4)2(β2)3 subtype than the corresponding (1S, 5R)-form ent-
No References for this article.
No Supplementary Data.
Document Type: Research Article
Institut für Pharmazeutische Chemie der Philipps-Universität Marburg, Marburg, Germany
Institut für Pharmazeutische Chemie der Philipps-Universität Marburg, Marbacher Weg 6, Marburg, D-35032, Germany, Email: [email protected]
Publication date: 2004-06-01
More about this publication?
Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Ingenta Connect is not responsible for the content or availability of external websites