Home >> Expert Review of Molecular Diagnostics, Volume 5, Number 4

Automated chip-based device for simple and fast nucleic acid amplification

Authors: Münchow, Götz; Dadic, Dalibor; Doffing, Frank; Hardt, Steffen; Drese, Klaus-Stefan

Source: Expert Review of Molecular Diagnostics, Volume 5, Number 4, July 2005 , pp. 613-620(8)

Publisher: Expert Reviews

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Abstract:

A chip-based PCR device is presented that is capable of rapid temperature ramping and handling sample volumes in the microliter range. The PCR chip comprises a microchannel thermally connected to three temperature zones. Inside this microchannel, the PCR sample plug is driven and precisely positioned by a ferrofluidic actuator for more than 40 cycles within 5 min. Computer simulations predict that the sample plugs are thermally equilibrated on a time scale of some 10 ms when transported to a different temperature zone. Hence, the thermal limitations on the cycle speed of the system are considerably reduced compared with conventional cyclers. The system was developed on a modular platform suitable for handling further microfluidic tasks such as DNA extraction and preparation of the PCR mix. Thus, the aspired chip-based platform represents not only a PCR system but a complete analysis system, from the injection of a patient's blood sample to its final appraisal.

Keywords: lab-on-a-chip; microfluidics; modular system; PCR

Document Type: Research article

DOI: http://dx.doi.org/10.1586/14737159.5.4.613

Publication date: 2005-07-01

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