Authors: Viswanathan, Kasinath; Früh, Klaus

Source: Expert Review of Proteomics, Volume 4, Number 6, December 2007 , pp. 815-829(15)

Publisher: Expert Reviews

Abstract:

Viruses constantly adapt to and modulate the host environment during replication and propagation. Both DNA and RNA viruses encode multifunctional proteins that interact with and modify host cell proteins. While viral genomes were the first complete sequences known, the corresponding proteomes are only now elucidated, with some surprising results. Even more daunting is the task to globally monitor the impact of viral infection on the proteome of the host cell and many technical hurdles must still be overcome in order to facilitate robust and reproducible measurements. Further complicating the picture is the dynamic nature of proteins, including post-translational modifications, enzymatic cleavage and activation or destruction by proteolytic events. Nevertheless, several promising studies have been published using high-throughput methods directly measuring protein abundance. Particularly, quantitative or semiquantitative mass spectrometry-based analysis of viral and cellular proteomes are now being used to characterize viruses and their host interaction. In addition, the full set of interactions between viral and host proteins, the interactome, is beginning to emerge, with often unexpected interactions that need to be carefully validated. In this review, we will discuss two major areas of viral proteomics: first, virion proteomics (such as the protein characterization of viral particles) and second, proteoviromics, including the viral protein interactomics and the quantitative analysis of host cell proteome during viral infection.

Keywords: interactomics; iTRAQ™; LC-MS/MS; liquid chromatography; MALDI; matrix-assisted laser desorption/ionization; proteoviromics; SILAC; stable isotope labeling of amino acids in cell culture; tandem affinity purification; tandem mass spectrometry; TAP; virion proteomics; Y2H; yeast two hybrid

Document Type: Research article

DOI: http://dx.doi.org/10.1586/14789450.4.6.815

Publication date: 2007-12-01

More about this publication?

• Expert Review of Proteomics explores technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease.
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Terms & Conditions
• Information for Librarians
• Free Trials
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Biology
• By this author: Viswanathan, Kasinath ;  Früh, Klaus

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers