Clinical experience using cultured epithelial autografts leads to an alternative methodology for transferring skin cells from the laboratory to the patient
Authors: Hernon, Catherine A1; Dawson, Rebecca A; Freedlander, Eric; Short, Robert; Haddow, David B; Brotherston, Michael; MacNeil, Sheila
Source: Regenerative Medicine, Volume 1, Number 6, November 2006 , pp. 809-821(13)
Publisher: Future Medicine
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Abstract:
We report a 10-year audit using cultured epithelial autografts (CEAs) for patients with extensive burns. Clinical take using CEAs averaged only 45% (as has been reported by others) but over half of all cells cultured for these patients had to be discarded owing to difficulties of timing the production of CEA sheets to the needs of the patients. CEAs could not be used until they had reached confluence and formed an integrated sheet, which took, on average, 12 days. However, once formed, they needed to be used within 2-3 days or they lost the ability to attach to wound beds. In response to this we developed a simpler carrier dressing methodology for transferring cultured subconfluent keratinocytes from the laboratory to the wound bed. This methodology offers an increase in speed of delivery but its major contribution is the greater flexibility in timing the transfer of cells from the laboratory to the changing needs of the patients.Keywords: burns; cell delivery; cultured epithelial autografts; keratinocytes; Myskin™
Document Type: Research article
DOI: 10.2217/17460751.1.6.809
Affiliations: 1: 1Tissue Engineering Group, Department of Engineering Materials, Kroto Research Institute, University of Sheffield North Campus, Broad Lane, Sheffield, S3 7HQ, UK., Email: s.macneil@sheffield.ac.uk
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Click here for Page Help