Authors: Balgley, Brian M; Wang, Weijie; De Voe, Don L; Lee, Cheng S

Source: Personalized Medicine, Volume 4, Number 1, February 2007 , pp. 45-58(14)

Publisher: Future Medicine

Abstract:

There is an urgent need for the development of technologies that allow the monitoring of protein expression and processing in tumor tissues resulting from development, physiology and disease state. To address the issue of cell heterogeneity in the tissue section, several microdissection techniques have been developed to provide a rapid and straightforward method for isolating selected subpopulations of cells for downstream molecular analysis. Development and demonstration of an effective discovery-based proteome platform, Gemini, are particularly highlighted for its capabilities of achieving ultrasensitive and comprehensive analysis of minute proteins extracted from targeted cells in tissue specimens. The greatest expectations for targeted proteomics research using enriched nonmalignant and malignant cells from high-quality fresh-frozen, formalin-fixed and paraffin-embedded specimens reside in the identification of diagnostic, prognostic and predictive biological markers in the clinical setting, as well as the discovery and validation of new protein targets in the biopharmaceutical industry.

Keywords: biofluid; biomarker; cancer; capillary isoelectric focusing; clinical; liquid chromatography; mass spectrometry; microdissection; proteomics; tissue

Document Type: Research article

DOI: http://dx.doi.org/10.2217/17410541.4.1.45

Affiliations: 1: 1Calibrant Biosystems, 910 Clopper Road, Suite 220N, Gaithersburg, MD 20878, USA., Email: brian.balgley@calibrant.com

Publication date: 2007-02-01

More about this publication?

• Personalized Medicine translates recent genomic, genetic and proteomic advances into the clinical context. The journal provides an integrated forum for all players involved - academic and clinical researchers, pharmaceutical companies, regulatory authorities, healthcare management organizations, patient organizations and others in the healthcare community.
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Terms & Conditions
• Information for Librarians
• E-Access Trials
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Medicine ,  Genetics
• By this author: Balgley, Brian M ;  Wang, Weijie ;  De Voe, Don L ;  Lee, Cheng S

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers