Authors: Komoto, Satoshi; Taniguchi, Koki

Source: Future Virology, Volume 1, Number 6, November 2006 , pp. 833-846(14)

Publisher: Future Medicine

Abstract:

The rotavirus genome is composed of 11 segments of double-stranded (ds)RNA. Recent studies have elucidated the precise mechanisms in transcription and replication of rotavirus RNA mainly by in vitro experiments. However, the ideal methodology for the molecular study of rotavirus replication is reverse genetics, which enables the viral genome to be artifically manipulated. Since the development of the first reverse genetics system for RNA virus in bacteriophage QB in 1978, the methodology has been developed for a variety of RNA viruses with plus-strand, minus-strand or dsRNA as a genome. However, there have been no reports on the reverse genetics of the viruses in the family Reoviridae with a genome of 10-12 segmented dsRNA, except for reovirus. This review describes the replication cycle of rotavirus with the aim of providing a general background to the development of rotavirus reverse genetics, and summarizes the reverse genetics system for dsRNA viruses, including rotavirus.

Keywords: double-stranded RNA virus; Reoviridae; rescue of transfectant virus; reverse genetics; RNA segment; rotavirus; viral selection system

Document Type: Research article

DOI: http://dx.doi.org/10.2217/17460794.1.6.833

Publication date: 2006-11-01

More about this publication?

• Future Virology provides an interdisciplinary forum for all scientists working in the fields of virology and genomic research. The journal delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research.
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Terms & Conditions
• Information for Librarians
• E-Access Trials
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Medicine
• By this author: Komoto, Satoshi ;  Taniguchi, Koki

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers