Oxidative Stress and Apoptosis of Human Brain Microvascular Endothelial Cells Induced by Free Fatty Acids
Source: The Journal of International Medical Research, 1 November 2009, vol. 37, no. 6, pp. 1897-1903(7)
Abstract:Damage to endothelial cells is a key event in the pathogenesis of atherosclerosis and vascular disease. This study aimed to determine whether free fatty acids (FFAs) induced oxidative stress and apoptosis in human brain microvascular endothelial cells (HBMVECs) in vitro and, if so, which signalling pathway mediated these effects. After culture in different concentrations of FFAs for 24 – 72 h, cell viability/proliferation was determined using a cell counting kit, apoptosis was detected by measuring caspase-3 activity and by using annexin V-conjugated fluorescein isothiocyanate/propidium iodide staining, and oxidative stress was evaluated by measuring the levels of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP). The HBMVECs exposed to FFAs showed significantly decreased cell proliferation, increased apoptosis and ROS levels, and decreased MMP. In conclusion, the results showed that high levels of FFAs induced oxidative stress, which damaged HBMVECs and resulted in apoptosis.
Document Type: Research Article
Affiliations: 1: Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China; Department of Neurology, Changshu Hospital, Yangzhou University, Changshu, China 2: Department of Neurology, Shanghai 10th Hospital, Tongji University, Shanghai, China 3: Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China 4: Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China 5: Department of Neurology, 1st Hospital, Zhejiang University, Hangzhou, China