Anti-proliferative Effects of Oridonin on SPC-A-1 Cells and its Mechanism of Action
Authors: Liu, J.J.; Huang, R.W.; Lin, D.J.; Peng, J.; Wu, X.Y.; Pan, X.L.; Li, M.Q.; Lin, Q.
Source: The Journal of International Medical Research, Volume 32, Number 6, November 2004 , pp. 617-625(9)
Publisher: Field House Publishing
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Abstract:
Oridonin, an extract from the Chinese herb Rabdosia rubescens, is currently one of the most important traditional Chinese herbal medicines. We investigated the anti-proliferative effect of oridonin on the lung cancer cell line SPC-A-1 and its mechanism of action. Growth inhibition was measured using a microculture tetrazolium assay and apoptosis was measured by several standard methods. Western blot analysis measured the expression of bcl-2 and bax proteins. Oridonin (> 28 μmol/l) inhibited the growth of SPC-A-1 cells and induced apoptosis. Marked morphological changes indicative of apoptosis were observed, especially in cells treated with oridonin for 48 - 60 h. Western blot analysis revealed downregulation of bcl-2 and upregulation of bax proteins following treatment with oridonin for 48 h. We conclude that oridonin demonstrated anti-proliferative and apoptosis-inducing effects on SPC-A-1 cells in vitro, and that changes in bcl-2 and bax protein levels may play an important role in its mechanism of action.Keywords: ORIDONIN; CHINESE HERBAL MEDICINE; RABDOSIA RUBESCENS; CANCER; SPC-A-1 CELLS; APOPTOSIS; BCL-2; BAX
Document Type: Research article
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