Spatial Distribution of Nociceptive Neuropeptide and Nerve Growth Factor Depletion in Experimental Diabetic Peripheral Nervous System

Authors: Kanbayashi, H.; Itoh, H.; Kashiwaya, T.; Atoh, K.; Makino, I.

Source: The Journal of International Medical Research, Volume 30, Number 5, September 2002 , pp. 512-519(8)

Publisher: Field House Publishing

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Abstract:

This study investigated the time-course of the nociceptive neuropeptide substance P and nerve growth factor (NGF), which facilitates substance P production, in lumbar and cervical dorsal root ganglia (DRG) of streptozotocin-induced diabetic rats. Levels of substance P and NGF were measured by radioimmunoassay and sandwich enzyme-linked immunosorbent assay, respectively, 2 months, 4 months and 8 months after induction of diabetes, and compared with age-matched non-diabetic control rats. At 2 months and 4 months, substance P and NGF levels were lower in the lumbar DRG of the diabetic rats than in controls. At 8 months, substance P and NGF were lower in both the lumbar and cervical DRG of the diabetic rats than in controls. These data demonstrate that a decrease in substance P levels in primary sensory neurons with NGF depletion occurs in an axonal length-dependent manner in diabetic rats, and that this decrease may be correlated with the duration of diabetes.

Keywords: DIABETES; RATS; DORSAL ROOT GANGLIA; NERVE GROWTH FACTOR; SUBSTANCE P

Document Type: Research article

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