Authors: Alout, Haoues; Berthomieu, Arnaud; Cui, Feng; Tan, Yi; Berticat, Claire; Qiao, Chuanling; Weill, Mylène

Source: Journal of Medical Entomology, Volume 44, Number 3, May 2007 , pp. 463-469(7)

Publisher: Entomological Society of America

Abstract:

Insecticide resistance owing to insensitive acetylcholinesterase (AChE)1 has been reported in several mosquito species, and only two mutations in the ace-1 gene have been implicated in resistance: 119S and 331W substitutions. We analyzed the AChE1 resistance status of Culex vishnui (Theobald) and Culex tritaeniorhynchus Giles sampled in various regions of China. These two species displayed distinct mutations leading to AChE1 insensitivity; the 119S substitution in resistant C. vishnui mosquitoes and the 331W substitution in resistant C. tritaeniorhynchus. A biochemical test was validated to detect the 331W mutation in field samples. The comparison of the recombinant G119S and 331W mutant proteins produced in vitro with the AChE1 extracted from resistant mosquitoes indicated that the AChE1 insensitivity observed could be specifically attributed to these substitutions. Comparison of their biochemical characteristics indicated that the resistance conferred by these mutations depends on the insecticide used, regardless of its class. This resistance seemed to be fixed in the Cx. tritaeniorhynchus populations sampled in a 2,000-km transect, suggesting a very high level of insecticide application or a low fitness cost associated with this 331W mutation.

Keywords: Culex tritaeniorhynchus; Culex pipiens; insecticide resistance; acetylcholinesterase insensitivity

Document Type: Research article

Publication date: 2007-05-01

More about this publication?

• Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.
  
  The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distribution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents; Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention; Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research articles, the journal publishes interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
• Editorial Board
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Visit this journal's homepage
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Entomology
• By this author: Alout, Haoues ;  Berthomieu, Arnaud ;  Cui, Feng ;  Tan, Yi ;  Berticat, Claire ;  Qiao, Chuanling ;  Weill, Mylène

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers