Home >> Journal of Medical Entomology, Volume 42, Number 4

The Drosophila melanogaster cinnabar Gene Is a Cell Autonomous Genetic Marker in Aedes aegypti (Diptera: Culicidae)

Authors: Sethuraman, Nagaraja; O'brochta, David A.

Source: Journal of Medical Entomology, Volume 42, Number 4, July 2005 , pp. 716-718(3)

Publisher: Entomological Society of America

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Abstract:

The cinnabar gene of Drosophila melanogaster (Meigen) encodes for kynurenine hydroxylase, an enzyme involved in ommochrome biosynthesis. This gene is commonly included as a visible genetic marker in gene vectors used to create transgenic Aedes aegypti (L.) that are homozygous for the kh w allele, the mosquito homolog of cinnabar. Unexpectedly, the phenotype of cells expressing kynurenine hydroxylase in transgenic Ae. aegypti is cell autonomous as demonstrated by the recovery of insects heterozygous for the kynurenine hydroxylase transgene with mosaic eye color patterns. In addition, a transgenic gynandromorph was recovered in which one-half of the insect was expressing the kynurenine hydroxylase transgene, including one eye with red pigmentation, whereas the other half of the insect was homozygous kh w and included a white eye. The cell autonomous behavior of cinnabar in transgenic Ae. aegypti is unexpected and increases the utility of this genetic marker.

Keywords: Aedes aegypti; kynurenine hydroxylase; cinnabar; khw; piggyBac

Document Type: Short communication

Publication date: 2005-07-01

More about this publication?
  • Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

    The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distribution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents; Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention; Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research articles, the journal publishes interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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