Analysis of ITS2 DNA Sequences from Brazilian Anopheles darlingi (Diptera: Culicidae)

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Specimens of Anopheles darlingi Root, the major vector of malaria in Brazil, were collected from several states in Brazil: Sao Paulo (Dourado), Bahia (Itabela), Rondônia (Porto Velho), Roraima (Boa Vista), and Acre (Plácido de Castro). Sequence divergence in the 2nd internal transcribed spacer (ITS2) was examined. The ITS2 sequences of mosquitoes captured in the Amazon region (Porto Velho, Boa Vista and Plácido de Castro) and in the northeast of Brazil (Itabela) were almost identical; however, a 4–5% sequence divergence was observed in the ITS2 of mosquitoes captured in the southeast (Dourado). Further analysis is needed to determine if these differences indicate that Dourado population may be a separate species.

Keywords: Anopheles darlingi; polymorphism; ribosomal DNA; second internal transcribed spacer

Document Type: Short Communication

Publication date: September 1, 1999

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  • Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

    The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distribution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents; Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention; Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research articles, the journal publishes interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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