Detection of Escherichia coli O157:H7 from Musca domestica (Diptera: Muscidae) at a Cattle Farm in Japan

Authors: Iwasa, Mitsuhiro; Makino, Sou-Ichi; Asakura, Hiroshi; Kobori, Hideaki; Morimoto, Yo

Source: Journal of Medical Entomology, Volume 36, Number 1, January 1999 , pp. 108-112(5)

Publisher: Entomological Society of America

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Abstract:

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 was isolated for the first time from Musca domestica L. A total of 310 fly samples was collected from 4 different farms in Obihiro-City, Hokkaido, in the summer and autumn of 1997; 5 samples carried E. coli serotype O157:H7. Using ELISA and Vero cell cytotoxicity assays, 3 isolates from 1 cattle farm produced both active Shiga-toxin type 1 (Stx1) and 2 (Stx2). These isolates also carried hemolysin and eaeA genes and harbored the 90-kb virulence plasmid of EHEC O157:H7. Based on plasmid profiles, antibiotic patterns, polymerase chain reaction (PCR)-based DNA finger printing analysis using random amplified polymorphic DNA, pulsed field gel electrophoresis analysis, and DNA sequences of stx ₁ and stx ₂, all 3 isolates from fly samples were identical. These results indicate that the house fly is capable of carrying the toxigenic EHEC O157:H7 involved in human disease.

Keywords: enterohemorrhagic Escherichia coli O157:H7; Musca domestica; Shiga-toxin; polymerase chain reaction

Document Type: Research article

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