Home >> Journal of Medical Entomology, Volume 36, Number 1

Detection of Escherichia coli O157:H7 from Musca domestica (Diptera: Muscidae) at a Cattle Farm in Japan

Authors: Iwasa, Mitsuhiro; Makino, Sou-Ichi; Asakura, Hiroshi; Kobori, Hideaki; Morimoto, Yo

Source: Journal of Medical Entomology, Volume 36, Number 1, January 1999 , pp. 108-112(5)

Publisher: Entomological Society of America

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Abstract:

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 was isolated for the first time from Musca domestica L. A total of 310 fly samples was collected from 4 different farms in Obihiro-City, Hokkaido, in the summer and autumn of 1997; 5 samples carried E. coli serotype O157:H7. Using ELISA and Vero cell cytotoxicity assays, 3 isolates from 1 cattle farm produced both active Shiga-toxin type 1 (Stx1) and 2 (Stx2). These isolates also carried hemolysin and eaeA genes and harbored the 90-kb virulence plasmid of EHEC O157:H7. Based on plasmid profiles, antibiotic patterns, polymerase chain reaction (PCR)-based DNA finger printing analysis using random amplified polymorphic DNA, pulsed field gel electrophoresis analysis, and DNA sequences of stx 1 and stx 2, all 3 isolates from fly samples were identical. These results indicate that the house fly is capable of carrying the toxigenic EHEC O157:H7 involved in human disease.

Keywords: enterohemorrhagic Escherichia coli O157:H7; Musca domestica; Shiga-toxin; polymerase chain reaction

Document Type: Research article

Publication date: 1999-01-01

More about this publication?
  • Journal of Medical Entomology is published bimonthly in January, March, May, July, September, and November. The journal publishes reports on all phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of public health and veterinary significance.

    The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distribution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents; Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention; Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research articles, the journal publishes interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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